Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets

The regulation of glycerol permeability in the gastrointestinal tract is crucial to control fat deposition, lipolysis and gluconeogenesis. Knowing that amino acid glutamine a physiological regulator gluconeogenesis, whereas cystine promotes adiposity, herein we investigated effects dietary supplementation with on serum biochemical parameters piglets fed acid-enriched diets, as well transcriptional profile membrane water channels aquaporins (AQPs) ileum portion small intestine its impact intestinal permeability. Twenty male an initial body weight 8.8 ± 0.89 kg were allocated four treatments (n = 5) received, during week-period, basal diet without (control) or supplemented 8 kg/ton (Gln), (Cys) combination two acids equal proportions (Gln + Cys). Most found improved Gln Cys diet. mRNA levels AQP3 predominant over others. Both acids, individually combined, responsible for consistent downregulation AQP1 , AQP7 AQP10 impacting Conversely, enriched upregulated enhancing basolateral membranes downregulating kinase ( GK ) cells. Altogether, our data reveal can modulate AQPs expression unveil promising target adipogenesis regulation.